GAYATHIRI M, THIRIBHUVANAMALA G, KRISHNAMOORTHY A S, HARIPRIYA S, AKSHAYA S B, ARUMUKAPRAVIN I
003369 GAYATHIRI M, THIRIBHUVANAMALA G, KRISHNAMOORTHY A S, HARIPRIYA S, AKSHAYA S B, ARUMUKAPRAVIN I (Nanoscience and Technology Dep, Tamil Nadu Agricultural Univ, Coimbatore - 641 003, Tamil Nadu) : Characterization of antimicrobial metabolites from medicinal mushrooms against Mango anthracnose pathogen Colletotrichum gloeosporioides (Penz.) Sacc.. Annals Phytomed 2021, Vol 10 (1), 185 - 94.
Mango is prone to attack by many diseases and one among them is the anthracnose disease, caused by Colletotrichum gloeosporioides (Penz.) Sacc. which cause both pre and post-harvest yield losses even up to 100 per cent based on prevailing wet humid weather condition. The present investigation has been focused on exploring the possibilities of identifying bioactive compounds from macro basidiomycetes against C. gloeosporioides. In the present study, different solvents such as ethyl acetate, diethyl ether, hexane, and chloroform at different concentrations (0.1 to 1 per cent) were used to extract secondary metabolites from culture filtrates of medicinal mushrooms, Ganoderma lucidum and Auricularia. polytricha. However, the ethyl acetate extracts of G. lucidum and diethyl ether fraction of A. polytricha at 1 per cent concentration showed maximum mycelial growth inhibition of 68.55 per cent and 58.55 per cent, respectively. Characterization of antimicrobial compounds from ethyl acetate extracted culture filtrates of G. lucidum through gas chromatography-mass spectrometry (GC-MS) indicated the presence of novel compound papaverine which recorded the highest peak area, 2.13 per cent with a probability of 90.74 per cent at RT 26.68. Further, the functional groups identified through fourier transform infra-red spectrophotometer (FT-IR) analysis revealed the nature of compounds belonging to aliphatic primary amine, amine salt, thiol, amide, conjugated ketone, alkane, and alcohol. The thin layer chromatography (TLC) studies performed with the ethyl acetate extracted culture filtrate of G. lucidum indicated the appearance of two bands at the Rf value of 0.45 and 0.29. Further, testing of eluted bands through agar well diffusion assay showed 36.66 and 40.50 per cent inhibition of mycelial growth of C. gloeosporioides, respectively. The standard samples of papaverine (Source: Sigma Aldrich and Compound code: P3510-5g) tested against mycelial growth and conidial germination of C. gloeosporioides exhibited 100 per cent inhibition of mycelial growth of C. gloeosporioides at 1500 ppm and 3000 ppm in agar well diffusion assay and paper disc assay which confirmed the presence of antimicrobial metabolites from culture filtrates of G. lucidum.
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